中国古代木槿栽培应用发展史初探

木槿是中国传统的乡土花木,是栽培历史最悠久的花木之一。本文根据历史文献和诗歌等梳理分析了历史各个时期的木槿主要栽培应用史实和重要成果,分析了不同历史时期木槿的栽培应用、食用、药用与品种等发展演化情况,发现了诸多的习俗和应用被保留和传承,以期为木槿资源、文化及其产业的传承与发展提供思路。     

基于森林公园的新疆森林康养旅游资源空间分布及影响因素分析

基于新疆65个森林公园,以平均最邻近指数、变异系数、地理集中指数和基尼系数等对其空间分布和影响因素进行分析。结果表明,新疆森林公园分布类型为集聚性分布,且具有“北密南疏”的特点;其空间分布在省际空间范围内呈非均衡的集中分布,且分布均匀度较大;森林公园的分布与人口密度、交通通达度及经济发展水平重合度较低,与水资源分布重合度较高,具有一定配比性;部分公园周边景区及林果资源丰富。     

紫花槭秋季叶色表达转录组初步分析

为了丰富紫花槭转录组数据,进一步开展紫花槭秋季叶片呈色机制研究.本研究以紫花槭秋季转色期三个阶段(前期,中期,后期)叶片为材料,采用高通量测序技术进行转录组初步分析.转录组数据共获得50501条Unigene,有35316条Unigene在数据库中得到注释,其中NR数据库中注释到的Unigene数量最多,共35024条,占69.4％.在注释到的物种中,紫花槭比对的Unigene与甜橙(Citrus sinensis)相似度最高,共有4290条,占12.25％.紫花槭转录组中的Unigene根据GO功能可分为生物学过程、细胞组分和分子功能3大类,共有25375条,其中生物学过程的基因最多,主要聚集于代谢过程和细胞过程等.基于Unigene库的基因结构分析,其中SSR分析共获得12711个SSR标记,占Unigene总数的36％.SSR位点共包含150种重复基元,单碱基重复所占比例最高(7184个,61.86％),四碱基重复、五碱基重复和六碱基重复所占比例较低.Unigene库中共有328239个SNP位点,发生频率为1/190 bp,SNP位点分为转换和颠换两种类型的碱基替换方式,其中碱基转换位点213787个(65.13％),碱基颠换位点114452个(34.87％),碱基转换类型发生频率高于颠换类型.6种单碱基变异中,2种转换类型A/G、C/T的发生频率分别为33.03％和32.10％;4种颠换类型中A/T发生频率最高,为11.52％;C/G发生频率最低,为5.79％.紫花槭转录组秋季叶色表达的转录组分析,可为紫花槭叶色基因调控、定向分子育种和培育彩叶新品种提供研究提供基础的数据信息.     

甘草总黄酮对高脂条件下罗非鱼肝损伤的保护作用

为了研究甘草总黄酮对高脂饲料造成的罗非鱼(Oreochromis niloticus)脂肪肝损伤是否具有保护作用,随机将180尾健康无伤罗非鱼分成6组,即对照组、高脂饲料模型组和4个不同浓度甘草总黄酮组(0.05、0.10、0.50、1.00 g·kg^-1甘草总黄酮),每组30尾。饲喂90 d后,采集罗非鱼各实验组肝,测定肝匀浆中超氧化物歧化酶(SOD)活性、还原型谷胱甘肽(GSH)活性、丙二醛(MDA)含量、甘油三酯(TG)含量、总胆固醇(TC)含量、总抗氧化能力(T-AOC)水平,Western blotting法检测肝中核转录因子-κB P65(NF-κB P65)和核转录因子-κB C-Rel (NF-κB C-Rel)蛋白表达水平,RT-PCR法测定肝中微粒体甘油三酸酯转移蛋白(MTTP)和载脂蛋白B100(ApoB100)基因表达情况。结果表明:与对照组相比,高脂饲料模型组罗非鱼肝匀浆中TG、TC、MDA含量极显著(P<0.01)升高,GSH活性、SOD活性、T-AOC水平均极显著(P<0.01)降低;MTTP和ApoB100 mRNA表达水平降低(P<0.01或P<0.05);Western blotting结果显示,NF-κB P 65和C-Rel蛋白表达水平升高。饲料中加入甘草总黄酮后,与高脂饲料模型组相比,罗非鱼肝中GSH活性、SOD活性、T-AOC水平不同程度提高,TC、TG、MDA含量和NF-κB P65、C-Rel蛋白表达水平不同程度降低,MTTP和ApoB100的mRNA表达水平升高,以0.50 g·kg^-1甘草总黄酮组效果较好。说明甘草总黄酮对于罗非鱼脂肪肝损伤具有一定的保护作用,可以缓解高脂饲料造成的脂肪肝损伤。     

Transport of cadmium from soil to grain in cereal crops: A review

Due to rapid urbanization and industrialization, many soils for crop production are contaminated by cadmium(Cd), a heavy metal highly toxic to many organisms. Cereal crops such as rice, wheat, maize, and barley are the primary dietary source of Cd for humans, and reducing Cd transfer from soil to their grains is therefore an important issue for food safety. During the last decade, great progress has been made in elucidating the molecular mechanisms of Cd transport, particularly in rice. Inter-and intraspecific variations in Cd accumulation have been observed in cereal crops. Transporters for Cd have been identified in rice and other cereal crops using genotypic differences in Cd accumulation and mutant approaches. These transporters belong to different transporter families and are involved in the uptake, vacuolar sequestration, root-to-shoot translocation, and distribution of Cd. Attempts have been made to reduce Cd accumulation in grains by manipulating these transporters through overexpression or knockout of the transporter genes, as well as through marker-assisted selection breeding based on genotypic differences in Cd accumulation in the grains. In this review, we describe recent progress on molecular mechanisms of Cd accumulation in cereal crops and compare different molecular strategies for minimizing Cd accumulation in grains.     

基于PID直流电机驱动的移动式采摘机器人设计

为了提高移动式采摘机器人的控制效率和移动精度,将直流电机驱动电路引入到采摘机器人移动控制系统的设计中,并采用PID算法对移动速度进行调节,从而避免采摘机器人在移动过程中产生较大的速度波动,提高其作业时的平稳性。为了验证PID算法在采摘机器人移动控制系统上使用的可行性,模拟葡萄采摘机器人的作业环境,对采摘机器人移动过程的速度控制进行了仿真模拟,结果表明:达到速度预定目标时,PID算法比常规算法具有更快的响应速度,调节过程也更加平稳。     

SSCP Marker Development and Analysis of Candidate Restorer Gene Rf1 for Cotton Cytoplasmic Male Sterility

[Objective] Locating the cotton cytoplasmic male sterility (CMS) restorer gene Rf₁ is important for investigating restorer gene mechanisms and improving restorer lines. In our previous study, a gene cluster, with nine Pentatricopeptide repeat(PPR) genes and nine other genes, was found within the 160-kb Rf₁ target region in Scaffold 333. The objective here was to improve the density of Rf1-linked markers in the target region and determine the expression profiles of candidate genes. [Method] Using the sequences of the 18 genes, we designed 155 single-strand conformation polymorphism (SSCP) primers covering all of the gene sequences to identify the polymorphic SSCP markers between the fertile and sterile pools. Additionally, real-time polymerase chain reaction(PCR) was performed to analyze the expression profiles of eight candidate genes in the four developmental stages of buds of sterile, maintainer and restoring lines, respectively. [Result] In total, 15 polymorphic primers were identified. A genotype analysis of the F₂ population was conducted using the 15 primers and 3 other polymorphic simple sequence repeats (SSR) markers. The markers were distributed in a 4.8 cM range. In addition, owing to the influence of sterile cytoplasm or restorer genes, most of the genes showed different expression patterns in the four developmental stages of the three lines' buds. [Conclusion] SSCP markers tightly linked to Rf₁ were identified and the expression profiles of candidate genes were determined. This study provides a basis for the further fine mapping of restorer genes and for candidate gene screening.     

Straw mulching with fertilizer nitrogen: An approach for improving crop yield,soil nutrients and enzyme activities

Field experiments were conducted to study soil properties, soil enzymes activities, water use efficiency (WUE) and crop productivity after six years of soya bean straw mulching in the semi‐arid conditions of China. The experiment included four treatments: CK (Control), N (240 kg N ha^‐1), H (soya bean straw mulching at half rate 700 kg ha^‐1 with 240 kg N ha^‐1) and F (soya bean straw mulching at full rate 1,400 kg ha^‐1 with 240 kg N ha^‐1). Soil organic carbon (SOC), soil labile organic carbon (LOC), soil available N (AN), available P (AP) and enzyme activities were analysed after wheat harvesting in 2016 and 2017. Results show that straw amounts had positive effects on the soil fertility indices being higher for treatment F. The SOC, LOC, AN, AP and enzyme activities (i.e. saccharase, urease and alkaline phosphatase) were in the order of F > H > N > CK. High wheat grain yield and WUE were observed for F treatment. A total of six years mulching along with 240 kg ha^‐1 nitrogen fertilizer application is sufficient for wheat yield stability and improving soil properties except urease activities in the semi‐arid condition of China. However, the straw mulching amount should be further studied with minimum nitrogen fertilizer for an environment‐friendly and effective approach for improving the soil biological properties with adequate crop production on a sustainable basis in the semi‐arid region of China.     

“三系”杂交棉组合F1花粉育性与产量和品质的相关性研究

细胞质雄性不育“三系”（不育系、保持系和恢复系）制种因简化制种和节约劳动力成本已成为棉花杂种优势利用的重要手段。研究棉花花粉育性及“三系”组合F1可育花粉率与产量、品质性状的相关性，可以达到强化“三系”制种，提高育种效率的目的。用联苯胺-甲萘酚法测定12个不育系与4个恢复系杂交所得的48个F1的花粉育性，统计了可育花粉率并分析了可育花粉率与产量、品质的相关性。结果表明：－20℃保存条件下，花粉育性随贮藏时间的增加而减弱，51 h内其花粉育性依然保持88%以上，51 h内花粉育性无显著性影响。F1的可育花粉率均低于其恢复系及常规杂交种，大部分组合降低水平在20百分点以内。不同恢复系的恢复力有明显差异, 其中H46恢复力较强。F1的可育花粉率与单株铃数呈显著正相关。本研究结果有助于了解离体花粉的育性变化规律及“三系”杂交组合后代可育花粉率与产量品质的关系，可应用于异地授粉并在一定程度上指导“三系”杂交种选育，为育种工作提供一定的参考。     

历史街区街道界面景观要素及保护策略研究——以青岛鱼山历史文化街区为例

指出了历史文化街区是人类文明的“活化石”,是人类历史文化遗迹和日常生活的重要空间载体。历史文化街区的街道景观是街道展示景观的主要载体,通过对街道界面景观要素的提取、归纳与总结,得出街道界面景观要素由底界面、侧界面以及顶界面三大核心要素组成,并将其应用到鱼山历史文化街区街道景观内进行了分析研究,提出了一些保护策略。旨在进一步了解历史文化街区街道界面景观要素构成,为历史文化街区景观的保护提供更加清晰的思路。